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Healthy volunteers practicing the learned techniques exhibited profound increases in the release of epinephrine, which in turn led to increased production of anti-inflammatory mediators and subsequent dampening of the proinflammatory cytokine response elicited by intravenous administration of bacterial endotoxin.

 

Cyclic hyperventilation and retention: 20-30 breaths/min followed by empty lung breath retention for as long as comfortably possible.

Immediate and profound decrease of pCO2 and bicarbonate, and an increase in pH (reaching up to 7.75), indicating acute respiratory alkalosis, which normalized quickly after cessation of the breathing techniques. A significant decrease in oxygen saturation(50%). Increased epinephrine levels, increase in lymphocytes.the effects on epinephrine are likely a consequence of both the hyperventilation phase and hypoxia due to breath retention, as both have been demonstrated to increase epinephrine levels. The hyperventilation-induced increase in epinephrine was shown to be dependent on decreased levels of bicarbonate, as hyperventilation combined with bicarbonate infusion (resulting in hypocapnia and alkalosis, but normal bicarbonate levels) nullified epinephrine increase.

 

Study on C.H.A.R.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034215/

Note: This is the breathing method Wim Hof based his method on.

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